Species:

Human

Alternative Name:

ZEB1, Zinc finger E-box-binding homeobox 1, Transcription factor 8, TCF-8, NIL-2-A zinc finger protein, Negative regulator of IL2, AREB6, TCF8, BZP, DELTAEF1, FECD6, NIL2A, PPCD3, ZFHEP, delta-crystallin enhancer binding factor 1, Negative regulator of IL2, posterior polymorphous corneal dystrophy 3, TCF8BZP, represses interleukin 2 expression, ZEB, Zfhep, ZFHEP, ZFHX1A, Zfhx1a

Uniport ID:

P37275

Assay Type:

Sandwich

unit:

ng/mL

Standard:

10ng/mL

Sensitivity:

0.058 ng/mL

Detection Range:

0.16-10ng/mL

Assay Time:

3.5H

Sample Type:

Serum, Plasma, Saliva, Tissue homogenates, Cell lysates, Cell culture supernates and Other biological fluids；Sample Volume=100μL

Research:

Hepatic function

Test Principle:

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ZEB1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ZEB1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ZEB1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human ZEB1.You can calculate the concentration of Human ZEB1 in the samples by comparing the OD of the samples to the standard curve.